Evaluation of the initial manifestations of the toxic process in conditions of chronic action of low subtoxic doses of dioxins polluting the environment

AIM: To study the state of genome by indicators retrotransposon activity gene encoding DNA methyltransferase 1 (DNMT1); damage in animals from natural population bank vole (Clethrionomys glareolus) living vicinity a preserved landfill contaminated with low concentrations dioxins (landfill production and consumption waste "Salariyevo", Moscow).
 MATERIAL AND METHODS: The ERV-L, B1, L1 retrotransposons transcription level DNMT1 were evaluated real-time PCR. stability liver bone marrow cells was characterized comet assay method. Afterward, obtained characteristics (stability, reactivity, damage) response to environmental stress factors compared groups conditionally control samples.
 RESULTS: effects decrease classes B1 L1, an increase expression revealed voles under long-term chronic exposure doses revealed. An increased (on average up 56% tail comet) detected hepatocytes, addition winter effect small subtoxic dioxins.
 CONCLUSION: Suppression its epigenetic regulator (DNMT1) are regarded as adaptive responses polluting environment. alteration reactivity destabilization indicates launch initial mechanisms toxic process formation. created tested methodological base for opens prospects establishing threshold level, result, substantiating screening assessment local (territorial) risk public health biomonitoring.